Emadeldin Hassan E. Konozy Laboratory
Department of Biotechnology
Section : Proteomics and Glycobiology
Full Professor: Biochemistry
Head, Proteomics and Glycobiology Laboratory
Dean, Scientific Research Bureau
Head, Biotechnology Department
Africa City of Technology
4A/2 Blue Nile Bridge Ave, Alamlak, Bahri Central, Khartoum 11111, Sudan.
Tele: +249183799219, FAX: +249183799218.
Phone (Cell): +249 999104999
Email: ehkonozy@act.gov.sd
Plant lectin purification, characterization and their biological activity
The discovery of plant lectins represented a great impulse to current glycobiology researches. Lectins are protein or glycoprotein substances, usually of plant origin. They signify important biochemical components for several applications in the bio-chemical/medical arenas and in research. Sequence determinations and structural characterization assisted to resolve the mechanism of their action in many biological systems and processes. The discovery and characterization of many plant lectins and their specific action on glycan receptors led to explore their possible implementation in arrays of biological and medical applications. For example due to the narrow sugar specificity plant lectins can enter into cells, leading to cancer cells aggregation or agglutination and thereafter inhibit or prevent cancer cells growth. Antiulcer and Antioxidant Activity of many plant lectins have also been documented. Due to their ability to differentiate between normal and malignant cells the potential and the applicability of different plant lectins was investigated to be used as a drug targeting shuttle.
Proteomics of stressed plants
Proteomics is the large-scale study of proteins at their cellular level. Plants usually exposes to various biotic and abiotic types of stresses throughout their life cycle. The abiotic stresses comprise mainly drought, salt, temperature (low/high), flooding and food shortage/excess which hinder plant development and therefore the yield to a great degree.
Knowing the protein expressed by plant to adapt with such stresses will enable to engineer plant with great and improved adaptability to such harsh conditions and therefore enhance their crop yield and economic impact.
Experience
1996-2000 | PhD. | University of Pune, Pune, India |
1993-1996 | MSc. | University of Pune, Pune, India |
1990 – 1993 | BSc. | University of Pune, Pune, India |
2000-2002 | Post-doc | Faculty of Medicine, University of Sao Paulo, Ribeirao Preto, Brazil |
2002-2004 | Post-doc | Academia-Sinica, Taipei- Taiwan |
2007-2009 | Post-doc | Centre de la Researche Agronomique, Avignon, France |
2009- 2012 | Research Scientist | Africa City of Technology, Khartoum, Sudan |
2012-2014 | Teaching Staff | Libyan International Medical University, Benghazi, Libya |
2014-2015 | Teaching Staff | Faculty of medicine, Sirte University, Sirte, Libya |
2015 to now | Research Scientist | Africa City of Technology, Khartoum, Sudan |
Publications
[1] Ahmed, M. M. E., Hamed, M. E. A. and Konozy, E. H. E. Lectin Expression Patterns during Erythrina abyssinica and Abrus precatorius Seeds Germination: A Prelude towards Understanding the Enigmatic Biological Role of Plant Lectin. Journal of Biochemical Technology, 10, 3 2019), 40.
[2] Al Gaali, A., Modawe, G., Ahmed, R. M. and Konozy, E. H. E. Isolation of Jatropha Curcas Seeds Isolectins with Variable Affinity for Human and Animal Blood Types. Sudan Journal of Medical Sciences, 14, 4 2019), 202-209.
[3] Awadallah, A. K. E., Osman, M. E. M., Ibrahim, M. A., Bernardes, E. S., Dias‐Baruffi, M. and Konozy, E. H. E. Isolation and partial characterization of 3 nontoxic d‐galactose–specific isolectins from seeds of Momordica balsamina. Journal of Molecular Recognition, 30, 2 2017), e2582.
[4] Bhide, S. V., Kestwal, R. M., Konozy, E. H., Hsiao, C. D. and Roque-Barreira, M. C. Characterization of alpha-mannosidase from Erythrina indica seeds and influence of endogenous lectin on its activity2007).
[5] Dafalla, M. B., Konozy, E. and Saad, H. A. Biochemical and Histological Studies on the Effects of Azadrichta Indica Seeds Kernel Extract on Albino Ratsψ2012).
[6] E Konozy, E. H., Dafalla, M. B. and Hsiao, C.-D. Spectroscopic Studies on Erythrina lysistemon Seed Lectin Effect of Denaturing Agents on Lectin Quaternary Structure. Protein and peptide letters, 13, 6 2006), 581-586.
[7] Ghaieth, M. F., Elhag, S. R. M., Hussien, M. E. and Konozy, E. H. E. Antibiotics self-medication among medical and nonmedical students at two prominent Universities in Benghazi City, Libya. Journal of pharmacy & bioallied sciences, 7, 2 2015), 109.
[8] Ibrahim, M., Ahmed, S., Mohamed, M., Konozy, E. and Saeed, B. Purification and characterization of Momordica balsamina seed lectin and testing its modulating effects on gastric cancer commercial cell lines: Th17. Clinical Chemistry and Laboratory Medicine, 52, 11 2014).
[9] Ibrahim, M. A., Saeed, B. O., Ahmed, S. M., Konozy, E. M., Mohamed, M. E. and Elhag, A. K. Detection of Optimum pH of Momordica balsamina Seeds Lectin. Sudan Journal of Medical Sciences, 9, 4 2014), 217-220.
[10] Iesa, M. A. M., Awooda, H. A. and Konozy, E. H. E. Fractional exhaled nitric oxide along with a reduced force expiratory volume are conclusive prognostic biomarkers to alert for asthma exacerbation. Sudan Medical Monitor, 12, 1 2017), 1.
[11] Iesa, M. A. M., Awooda, H. A., Konozy, E. H. E. and Musa, O. A. A. Wheezing alone is not enough to validate asthma diagnosis among Libyan adults: A questionnaire-based study reinforced with pulmonary function test. Sudan Medical Monitor, 12, 1 2017), 25.
[12] Iesa, M. A. M., De Neini, O., Awooda, H. A. and Konozy, E. H. E. Serum Periostin Along With a Reduced Force Expiratory Volume Are Conclusive Prognostic Biomarkers to Alert for Asthma Exacerbation. Sudan Journal of Medical Sciences, 11, 4 2016).
[13] Iesa, M. A. M., Osman, M. E. M., Hassan, M. A., Dirar, A. I. A., Abuzeid, N., Mancuso, J. J., Pandey, R., Mohammed, A. A., Borad, M. J. and Babiker, H. M. SARS-CoV-2 and Plasmodium falciparum common immunodominant regions may explain low COVID-19 incidence in the malaria-endemic belt. New microbes and new infections, 382020), 100817.
[14] Iesa, M. A. M., Osman, M. E. M., Taha, M. H., Salah, E. T., Konozy, E. H. E., Hussein, S. E. and Mohamedani, A. A. Professionalism in Sudanese Medical Schools: A Situational Analysis as Perceived by Medical Graduates From 22 Medical Schools2021).
[15] Imad, S. A., Yasser, G. and Muhamed, E. H. bÜ| z| ÇtÄ TÜà| vÄx2014).
[16] Kestwal, R. M., Konozy, E. H. E., Hsiao, C.-D., Roque-Barreira, M. C. and Bhide, S. V. Characterization of α-mannosidase from Erythrina indica seeds and influence of endogenous lectin on its activity. Biochimica et Biophysica Acta (BBA)-General Subjects, 1770, 1 2007), 24-28.
[17] Konozy, E. H. and Bhide, S. V. Towards a better understanding of the enigmatic biological function of plant lectin: detection and identification of endogenous receptors for Erythrina indica seeds lectin. Macromol Ind J, 8, 2 2012), 48-56.
[18] Konozy, E. H., Sawhney, S. and Bhide, S. V. An approach towards understanding the physiological role of lectins: Detection and Identification of identification of endogenous receptors for Erythrina indica seed lectin. WISSENSCHAFTLICHE VERLAG MBH BIRKENWALDSTRASSE 44, POSTFACH 10 10 61, 70009 …, City.
[19] Konozy, E. H., Sawney, S. and Bhide, S. B. An approach toward understanding the physiological role of lectins: Detection and identification of endogenous receptors for Erythrina indica seed lectin. Eur J Cell Biol, 74, suppl 46 1997), 4.
[20] Konozy, E. H. E. Characterization of a D-Galactose-binding lectin from seeds of erythrinalysistemon. Turkish Journal of Biochemistry, 12012), 7-18.
[21] Konozy, E. H. E. Purification and Characterization of Acid Phosphatase (AcPase) from Cotyledon of Erythrina indica. Glob. Adv. Res. J. Agric. Sci, 52016), 195-203.
[22] Konozy, E. H. E. Isolation, Purification and Characterization of a Thermostable β-Galactosidase isoform from Erythrina indica Seeds. Global Advanced Research Journal of Agricultural Science, 5, 6 2016), 175-182.
[23] Konozy, E. H. E., Bernardes, E. S., Rosa, C., Faca, V., Greene, L. J. and Ward, R. J. Isolation, purification, and physicochemical characterization of a D-galactose-binding lectin from seeds of Erythrina speciosa. Archives of Biochemistry and Biophysics, 410, 2 2003), 222-229.
[24] Konozy, E. H. E., Causse, M. and Faurobert, M. Glycosidases Activities and Protein Content Variations during Fruit Development and Ripening in three Texture Contrasted Tomato Cultivars.
[25] Konozy, E. H. E., Causse, M. and Faurobert, M. Cell wall glycosidase activities and protein content variations during fruit development and ripening in three texture contrasted tomato cultivars. Saudi journal of biological sciences, 19, 3 2012), 277-283.
[26] Konozy, E. H. E., Dafalla, M. B. and Hsiao, C.-D. Spectroscopic Studies on Erythrina lysistemon Seed Lectin. Protein & Peptide Letters, 13, 586 2006), 58.
[27] Konozy, E. H. E., Mulay, R., Faca, V., Ward, R. J., Greene, L. J., Roque-Barriera, M. C., Sabharwal, S. and Bhide, S. V. Purification, some properties of a D-galactose-binding leaf lectin from Erythrina indica and further characterization of seed lectin. Biochimie, 84, 10 2002), 1035-1043.
[28] Konozy, E. H. E., Osman, M. E. M. and Iesa, M. A. M. SARS-CoV-2 and Plasmodium falciparum are probably adopting Analogous strategy to invade erythrocytes. Journal of infection and public health, 14, 7 2021), 883.
[29] Konozy, E. H. E., Rogniaux, H., Causse, M. and Faurobert, M. Proteomic analysis of tomato (Solanum lycopersicum) secretome. Journal of plant research, 126, 2 2013), 251-266.
[30] Konozy, E. H. E., Sabharwal, S. and Bhide, S. V. Influence of the intracellular localization of Erythrina indica seed acid phosphatase and beta-galactosidase on their interactions with the endogenous lectin. OXFORD UNIV PRESS INC JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA, City.
[31] Konozy, E. H. E. M. A. Bio_chemical studies on Erythrina indica lectin and its endogenous glycosidase receptors1999).
[32] Mohamed, R. A. E. H., El Rayah, E. A. and Konozy, E. Rift Valley Fever Virus (RVFv) Dissemination inside Mosquitoes and Investigation of the Influence of Climate on Mosquitoes Abundance2013).
[33] Osman, M. E., Awadallah, A. K. and Konozy, E. H. Isolation purification and partial characterization of three lectins from Tamarindus indica seeds with a novel sugar specificity. Int J Plant Res, 6, 1 2016), 13-19.
[34] Osman, M. E. M. and Konozy, E. H. E. Insight into Erythrina Lectins: Properties, Structure and Proposed Physiological Significance. The Open Bioactive Compounds Journal, 5, 1 2017).
[35] Santiago, F. M., Bernardes, E., Konozy, E., Roque-Barreira, M. C. and Mineo, J. R. N-acetyl-beta-D-glucosaminidase activity of a hydrophilic fraction from Toxoplasma gondii. OXFORD UNIV PRESS INC JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA, City.
[36] Siddig, W., Salim, M., Mohamed, A., Ibrahim, I., Farid, M. and Konozy, E. Regardless of the antibiotic group or type, misuse of antibiotics adversely impair liver, kidneys and heart functions “biochemical and histopathological assessment”. Am J Pharm Health Res, 22014), 73-94.
[37] Taha, S. A. H., Osman, M. E. M., Abdoelkarim, E. A. A., Holie, M. A. I., Elbasheir, M. M., Abuzeid, N. M. K., Al-Thobaiti, S. A., Fadul, S. B. and Konozy, E. H. E. Individuals with a Rh-positive but not Rh-negative blood group are more vulnerable to SARS-CoV-2 infection: demographics and trend study on COVID-19 cases in Sudan. New microbes and new infections, 382020), 100763.
[38] Yeh, Y.-H., Kesavulu, M. M., Li, H.-m., Wu, S.-Z., Sun, Y.-J., Konozy, E. H. E. and Hsiao, C.-D. Dimerization is important for the GTPase activity of chloroplast translocon components atToc33 and psToc159. Journal of Biological Chemistry, 282, 18 2007), 13845-13853.
[39] Yusria Elshiekh Abdalrahim, Isam Mohamed Abu Zeid, Saed A Al-Thobaiti and Emadeldin Hassan Elsaid Konozy, 2020. Isolation and Characterization of three Thermostable Acid Phosphatase isozymes from Cassia occidentalis (Coffee Senna) Seeds. Asian Journal of Plant Sciences, 19: 455-462.
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